Technology Information  Title One Step RT-PCR for Quantifying Small RNAs Manager Zhenyan Yan Case Number 07036 Abstract Researchers are only beginning to understand the mechanisms by which microRNAs (miRNA) and small interfering RNAs (siRNA) control gene expression. The increasing interest in this field of study has created a demand for tools to accurately and reliably detect miRNA and siRNA in biological samples. NC State researchers have developed a one-step RT-PCR method for the detection and quantification of miRNA and siRNA. This method is more sensitive and specific than the widely used microarray and Northern Blot techniques and readily lends itself to high throughput applications. Advantages Safe: a one step RT-PCR method that, unlike Northern Blot, does not require the use of radioisotopes. Sensitive: capable of detecting as little as 100 pg of total RNA per reaction, as compared to microarray techniques which require mg quantities. Specific: this method can discriminate between small RNA molecules differing by as few as one nucleotide. Flexible: this method is cost-effective for small-scale analyses but can easily be adapted for high throughput applications. Taxonomy NCSU Technology Categories/Diagnostic NCSU Technology Categories/Research Tool NCSU Technology Categories/PCR/Research Tool NCSU Technology Categories/RNA/Research Tool NCSU Technology Categories/RNAi/Research Tool Keywords siRNA, miRNA, RT-PCR  Web Links  Name Open Link Inventor's Laboratory Homepage Open Link Request Information If you are a registered user, please login before requesting information by clicking the Login button. If you have not registered, click the Login button for registration information.